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ktrC [2017-07-03 14:36:41]
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ktrC [2017-07-03 14:36:41]

low affinity potassium transporter KtrC-KtrD, peripheric membrane component
locus
BSU14510
pI
5.63
mw
24.20 kDa
protein length
221 aa Sequence Blast
gene length
663 bp Sequence Blast
function
potassium uptake
product
low affinity potassium transporter KtrC-KtrD, peripheric membrane component (proton symport)
essential
no
synonyms
ylxV,yzaC,ykqB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,520,531 → 1,521,196

    The protein

    Paralogous protein(s)

  • [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]

    • [SW|Domains]

  • contains a [SW|RCK_N domain] at the N-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+N-terminal+domain&sort=score UniProt])
  • contains a c-di-AMP-binding [SW|RCK_C domain] at the C-terminus (according to [http://www.uniprot.org/uniprot/?query=RCK+C-terminal+domain&sort=score UniProt]) [Pubmed|23671116]

    • Effectors of protein activity

  • the protein binds c-di-AMP [Pubmed|23671116]

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex, 56% identity) [Pubmed|23598340]
  • [PDB|4XTT] (the ''S. aureus'' KtrA [SW|RCK_C domain] in complex with c-di-AMP, 57% identity) [Pubmed|25957408]

    • [SW|Localization]

  • peripheral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]
    description
    [Pubmed|12562800]

    regulation

  • constitutively expressed [Pubmed|12562800]
    • view in new tab

    genes
    [gene|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC]-[gene|22DBF4D1F93519A95E6BCC0FB1AC2387DF444E27|ykqA]-[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002614], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|8002615], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • constitutively expressed [Pubmed|12562800]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A904 (ykqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/904 NBRP B. subtilis, Japan]
  • GHB6 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''spec''), [Pubmed|12562800] (available in [SW|Erhard Bremer]'s and [SW|Jörg Stülke]'s) labs, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A955&Search=1A955 BGSC] as 1A955
  • GP2264 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''aphA3''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2048 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2079 ([gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]::''tet''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
  • GP2083 (D([gene|92D0275E0768780F782F4B3724BC5181E436B6B1|ktrA]-[gene|A287B9771A56D03ADCA603C1969CBF03DCB10E95|ktrB])::''aphA3'' D''[gene|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|ktrC]''::''tet''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2907 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 25869574,27935846

    • Original publications

  • 12562800,23671116,23598340,28420751,28504641